Archive for the ‘CO2 Incubator’ category

CO2 Carbon Dioxide Monitor and Controller

November 29th, 2014

Acmas Technologies CO2 carbon dioxide monitor and controller assess indoor air quality and is an ideal instrument to monitor and keep control over CO2 being released by various processes.

This monitor is designed to provide the operator with a fast response measurement. The housing has been carefully shaped to fit comfortably in either hand. Itis manufactured utilizing high grade raw materials and is available in the market at a diminutive price range. We offer an exclusive range of carbon dioxide monitors to our customers. Our global clients have appreciated the wide variety of monitors catered by us provide near scientific accuracy, high reliability and functionality at an affordable price.


  • No electrical outlet needed
  • Alarm for high CO level
  • Low running and maintenance cost
  • High reliability – robust design
  • Simple installation – advanced alignment features
  • simple to use
  • data logging available
  • adjustable alarm
  • portable
  • rechargeable battery
  • User friendly setting buttons
  • Low power consumption


  • Food Packaging
  • Pharmaceutical research, production, and packaging
  • Purge Verification and contamination control
  • Biotechnology
  • Suitable for prevention and monitoring of CO2 potential leak and a Controller of Ventilation System in Below Applications Human Environment, Industrial Environment, Underground Parking Lot and Freezing machine room.

 Find here:- CO2 Carbon Dioxide Monitor

Technical specifications

     Providing up to three 0~10V analog output or up to three relay  dry-contact outputs
     The analog output with two alternatives
     Lineari zed over full range output
     PID control output
     User selectable ranges of CO2:          0 ~ 20,000 ppm,  0 ~     50,000
     Adjustable Range of Temperature:      5 – 45
     Adjustable Range of Humidity:           5~95% RH&
     RS-485Communication Interface                                  (optional )
     15KV Antistatic Protection
     Independent Base Address Setting

CO2 Incubator and In Vitro Fertilization (IVF)

April 24th, 2014

The desire to have children is a fundamental hope for most couples in fulfilling their life’s plans but this can be very stressful for couples affected by fertility. IVF is an assisted reproductive technology procedure where fertilization occurs “outside of the body in an incubator under controlled conditions”.

In Vitro Fertilization is used when female is the primary cause of the infertility (Endometriosis, Tubal disease, Hormonal dysregulation and Polycystic ovary syndrome) and Intracytoplasmic Sperm Injection (ICSI) is used in case of male infertility (if the quality of man sperms is inadequate).

“Incubators are vital to the overall success of IVF. They create the optimal, constant environment for embryo cultures. Good embryo culture conditions and subsequent embryonic development are dependent upon a correctly maintained incubator temperature and pH.

The most commonly used environmental conditions for human IVF incubators are 5% CO2 in air, 37°C, and 100% relative humidity. CO2 Incubators are the most important equipment for the IVF because the eggs harvested from the ovaries of the patients for fertilization spend most of the time outside the body that is in the incubator. Because this unit gives the special environment conditions of the body like 37°C (body temperature) and 5-7 % CO2 maintain the physiological pH value of the cultural medium used and offer the best conditions for optimal oocyte and embryo development.

CO2 is used to regulate the pH level, thus pH is measured and maintained by monitoring and adjusting the concentration of CO2. CO2 and pH have an inverse relationship; as the concentration of CO2 increases, pH level decreases.

How does IVF works?

In vitro fertilisation is a three stage procedure involving

  • Collecting eggs and sperms; an initial phase of injections to stimulate the growth of multiple eggs (typically 12 to 14 eggs are obtained in each cycle). The eggs are then collected through the vagina using ultrasound. This procedure is best performed under a light anaesthetic. The male partner usually would produce sperm on the same day although depending on the individual circumstances sperm can be kept frozen at the sperm bank or clinic. In IVF each egg is then placed in a droplet of culture fluid containing all the nutrients required for embryo development and approximately 50,000 sperm are added to each egg.
  • Incubation; The sperm and eggs are then cultured overnight in an incubator mainly CO2 Incubator.
  • Collection of fertilized eggs; eggs are examine for the sign of fertilization. The fertilised eggs are allowed to develop for a further 48 hours until they reach the day 3 stage when one anticipate that the best embryos will have between 6 and 8 cells..

Embryo transfer is a very important process in which the best embryos are placed in mother’s uterus. Pregnancy rates during IVF treatment is dependent on a number of factors such as the women’s age, hormone levels, associated gynecological conditions such as endometriosis, fibroids, ovarian cysts and pre-existing medical conditions.

CO2 Incubator

April 24th, 2014

As it is very well known that incubator maintains optimal temperature, humidity and other conditions such as Carbon dioxide (CO2) and oxygen content of the atmosphere inside. CO2 incubator controls the CO2 level to 5% and it works on three building blocks

  • Carbon dioxide
  • Temperature
  • Relative Humidity

CO2 incubators are usually set to 37°C. This is the optimal working temperature for promoting healthy cell growth. The external indicators of the incubator do often provide a good sense of the internal environment, but it is often useful to keep a good thermometer inside to keep a record of the internal temperature.

The chambers of CO2 incubators are sometimes incorporated with copper which is used for eliminating the contamination in incubators, as copper breaks down it releases copper oxide which destroy microbes that are present in the chamber.

CO2 incubator may be

  • Water-jacketed; envelopes the unit’s chamber and provides superior insulation while eliminating contamination and cold spots.The water-jacket is also surrounded by insulation, which makes the incubator ideal for maintaining precise conditions.
  • Air-jacketed; an air-jacket CO2 incubator has a heated inner chamber that is controlled by sophisticated microprocessors. These microprocessors constantly monitor and adjust program settings and allow calibrations of CO2 and temperature.

For both types of incubators, the microcomputer controls include backup systems that prevent samples from overheating, plus alarms that monitor the out-of-tolerance conditions.

Maintaining the chamber’s environment (CO2 control)

To maintain the chamber environment for safe and reliable research, CO2 incubators are equipped with precise automated controls. These controllers are of three types.

  • Continuous Flow; provides manual control of the air and CO2 flow rate through the use of precision-bore glass tube flow meters. For this method an accessory air pump is required.
  • Thermal Conductivity (TC)sensor ; more indirect method of CO2 detection and control since it measures the level of CO2 by sensing temperature differences as CO2 is introduced into the chamber.
  • Infrared (IR); This method uses an infrared sensor to detect a change in CO2 concentrations as little as 0.1 %. Since the IR sensor is unaffected by changes in temperature and humidity, it is ideal for applications in which the incubator door is frequently opened, and provides nearly perfect culturing conditions for cell growth. The sensor continuously samples chamber atmosphere through a spectrophotometer flow cell, checking wavelength and instantly correcting an out-of-control condition. Also, CO2 recovery is rapid and changes in CO2 concentration are made within seconds.

Areas of Application

  • Tissue Culture
  • Food and dairy processing
  • Stem cell research
  • Biochemical, bacteriological and hematological studies
  • In vitro fertilization
  • Virological studies

Safety Guidelines

  • Incubator should be located on a solid work surface that should be away from high traffic areas and extreme temperatures (Constant temperature is easier to maintain if the incubator is not in the direct contact of airflow from air conditioners or heat sources.
  • Electrical cord of the incubator should be routinely inspected for any damage (Electrical cord should not be used for cord repair).
  • This thermometer must also be cleaned with 70% alcohol on a regular basis to maintain its integrity.
  • The internal humidity of the incubator is usually maintained, by a tray of water which is kept topped up on a weekly basis. It is essential to always use sterile distilled water to do this.  The tray which holds the water must also be cleaned to prevent the expensive and time-consuming results of contamination by unwanted invaders. The incubator should be cleaned with 70% alcohol between uses and internal removable parts should be autoclaved to achieve full sterilisation.