Biosafety Cabinet Class II Manufacturers, Exporters & Suppliers in India

BIOLOGICAL SAFETY CABINET CLASS 2

What Is a Biological Safety Cabinet Class II?

Working with infectious agents, biohazardous materials, or sensitive biological cultures is not something you do casually. Every time a researcher or technician opens a sample inside an unprotected workspace, three things are at risk simultaneously: the person doing the work, the sample itself, and the environment around them. The standard that laboratories worldwide have arrived at for managing all three of these risks together is the Biological Safety Cabinet Class II, also referred to as a Biosafety Cabinet Class II or simply a BSC Class 2.

A Biological Safety Cabinet Class II is a ventilated, open-front laboratory enclosure that uses HEPA or ULPA-filtered vertical laminar downflow air and a defined inward airflow at the front aperture to create three simultaneous protective barriers: personnel protection from aerosol exposure, product protection from cross-contamination, and environmental protection through filtered exhaust. Unlike a Class I cabinet, which only protects personnel and the environment, the Class II design protects the sample as well, which is why it is the cabinet of choice for cell culture, microbiology, pharmaceutical compounding, and biosafety level 1, 2, and 3 work.

At ACMAS Technologies, under our manufacturing brand WEIBER, we design, manufacture, supply, and export Biosafety Cabinets Class II in all four sub-types (A1, A2, B1, and B2) to laboratories, pharmaceutical companies, hospitals, and research institutions across India and around the world. As experienced biosafety cabinet manufacturers and suppliers, we understand that the difference between a cabinet that merely meets a standard and one that genuinely protects your people and your work lies in engineering precision, material quality, and consistent manufacturing discipline.

The Four Types of Biosafety Cabinet Class II Explained

Not all Biosafety Cabinet Class II units are the same. The four sub-types differ significantly in their airflow management, exhaust requirements, and suitability for specific materials.

Class II Type A1 does not require external ducting, making it suitable for laboratories where exhaust ducting is not possible. It operates at a minimum inflow velocity of 75 ft/min, recirculates 70% of air through the supply HEPA filter, and exhausts the remaining 30% through the exhaust HEPA/ULPA filter. It is suitable for low-to-moderate risk biological agents but not for work with volatile toxic chemicals unless externally ducted.
Class II Type A2 must be externally vented. It operates at a minimum inflow velocity of 100 ft/min and exhausts 70% of cabinet air while recirculating 30%. A negative pressure plenum surrounds all contaminated positive-pressure plenums, providing an additional safety margin. It is suitable for work with small quantities of toxic chemicals and trace radionuclides as an adjunct to microbiological studies.
Class II Type B1 must be externally vented with 70% of air exhausted and 30% recirculated. Minimum inflow velocity is 100 ft/min. It is suitable for work with toxic chemicals and radionuclides when work is conducted in the directly exhausted portion of the cabinet.
Class II Type B2 is a total exhaust cabinet, 100% of air is exhausted through a dedicated external duct with no recirculation. It is designed for work with toxic chemicals, volatile radionuclides, and etiologic agents requiring maximum containment. Minimum inflow velocity is 100 ft/min.

How a Biosafety Cabinet Class II Works

Ambient room air is drawn through the front grille, creating an inward airflow that acts as the first barrier against aerosol escape. This inflow air joins approximately half of the recirculated downflow air to form a front air curtain that is precisely calibrated to maintain containment across the full working aperture. The combined airstream travels up through the rear column to the blower assembly at the top of the cabinet. A portion of this air is exhausted through the exhaust HEPA/ULPA filter. The remainder passes through the supply HEPA/ULPA downflow filter and returns to the work surface as a vertical laminar flow, creating an ISO Class 3 work environment that prevents cross-contamination between samples. The dual-wall construction surrounds the entire work zone with negative pressure plenums, so even in the event of a cabinet seal failure, contaminated air cannot escape to the room.

Key Features of ACMAS Biosafety Cabinets

Every Biological Safety Cabinet we manufacture is equipped with microprocessor-based PLC and HMI controls, an alphanumeric LCD display panel showing real-time airflow and filter status, airflow and lighting interlock for operational safety, a spare extraction fan for long exhaust ducting runs, ultraviolet UV sterilization lighting combined with fluorescent illumination exceeding 800 lux on the work surface, noise levels below 65 dB, corrosion-resistant stainless steel or powder-coated PCRC interiors and exteriors, and powder coating finish that actively prevents microbial growth on exterior surfaces. Both table-top and floor-standing configurations are available across four standard chamber sizes, and we manufacture to custom dimensions where required.

Custom-Built Biosafety Cabinets to Your Exact Specifications

Our standard models cover the most common laboratory requirements. However, we fully understand that specific research protocols, facility configurations, regulatory requirements, or procurement specifications sometimes demand something beyond a catalogue item. Our engineering team will design and manufacture Biosafety Cabinets Class II to your exact technical specifications, including non-standard chamber dimensions, specific material grades such as SS-304 or SS-316 interiors, particular control interface requirements, compliance with specific international standards including NSF/ANSI 49, EN 12469, ISO 14644-1, and IEC 61010-1, and integration with facility exhaust systems. This customisation capability is why procurement teams at pharmaceutical majors, government defence laboratories, and hospital networks consistently specify ACMAS when their requirements cannot be compromised.

Why Choose ACMAS as Your Biosafety Cabinet Manufacturer?

As a trusted biosafety cabinet manufacturer, supplier, and exporter in India, ACMAS combines decades of cleanroom and contamination control equipment manufacturing experience with in-house engineering capability, CE and ISO-certified quality systems, and a genuine commitment to delivering equipment that performs correctly in real working conditions. We supply standard units for straightforward needs and engineer fully customised solutions for complex requirements, both backed by installation support and after-sales technical assistance that continues well beyond delivery.

If you are sourcing a Biological Safety Cabinet Class II for a new laboratory, a pharmaceutical facility, a hospital, or a research institution, we invite you to share your specifications, room layout, and biosafety level requirements with us. Our team will recommend the right type and configuration, and get you a detailed, competitive quote promptly and without obligation.

It is classified in 4 categories:

Class II, type A1: this does not have to be vented, which makes it suitable for use in laboratory rooms which cannot be ducted. This cabinet is acceptable for use of low to moderate risk agents in the absence of volatile toxic chemicals and volatile radionuclides.

Type A, has a minimum inflow velocity of 75 ft/min. The filtered makeup air is divided equally over the work surface at about two to six inches above the work surface. Exhaust is drawn at the bottom of the cabinet where it rises to the top. At the top of the cabinet, 70% of the air recirculates through the supply HEPA filter, the other 30% of air exhausted through the exhaust HEPA/ULPA filter.

This type is not safe for work with hazardous chemicals except when ducted, usually with a "thimble" or canopy hood to avoid disturbing internal air flow.

Class II, type A2: this must be vented. 70% of the air is exhausted from the cabinet while 30% is recirculated. This cabinet may be used with etiologic agents treated with minute quantities of toxic chemicals and trace quantities of radionuclides that will not interfere with work if recirculated in the down flow air.

The Type A2 cabinet, has a minimum inflow velocity of 100 ft/min. A negative air pressure plenum surrounds all contaminated plenums that are under positive pressure. In other respects, the specifications are identical to those of a Type A1 cabinet.

Class II, type B1: this cabinet must be vented, with 30% of the air exhausted from the cabinet while 70% is recirculated back into the room. This cabinet may be used with etiologic agents treated with minute quantities of toxic chemicals and trace amounts of radionuclides required as an adjunct to microbiological studies if work is done in the directly exhausted portion of the cabinet, or if the chemicals or radionuclides will not interfere with the work when recirculated in the down flow air.

The Type B1 cabinets have a minimum inflow velocity of 100 ft/min

Class II, type B2: this cabinet must be totally exhausted, with100% of the air exhausted through a dedicated duct. This cabinet may be used with etiologic agents treated with toxic chemicals and radionuclides required as an adjunct to microbiological studies.
The Type B1 cabinets have a minimum inflow velocity of 100 ft/min.

NSF / ANSI 49
EN 12469
JIS K 3800
SFDA YY-0569
ISO 14644.1
JIS B9920
JIS BS5295
EN-1822 (H14
IEST-RP-CC001.3
IEST-RP-CC007
IEST-RP-CC034.1
UL-C-61010A-1
CSA22.2, No.1010-192
EN-61010-1
IEC61010-1

Airflow and lighting interlock
Alphanumeric LCD screen displays
Spare extraction fan for long exhaust ducting
High reliability
Low maintenance
Floor Standing and table top models available.
Microprocessor based control(HMI+PLC optional)
Reliable
Precise Control of Environmental
Aesthetically designed
Corrosion Resistant interior and exteriors
Sturdy Construction

The biosafety cabinet class 2are fabricated with inward air flow to protect personnel, HEPA-filtered downward vertical laminar airflow for product protection, and HEPA filtered exhaust air for environmental protection. When properly maintained and operated, they effectively contain and capture microbial contaminants and infectious agents using HEPA/ULPA filters.

Ambient air is pulled through front grille to create inflow, without going into the work surface. Inflow is joined by half of the downflow, to create front air curtain that is fine-tuned to create a large performance envelope. The combined air stream travels through the back air column towards the blower.

Approximately required air in the common plenum is exhausted through the HEPA/ULPA filter to the room. The remaining air is passed through the downflowHEPA/ULPA filter and into the work area as a vertical laminar flow air to create ISO Class 3 work surface and prevents cross contamination.

The biosafety cabinet class II has dual wall construction that surrounds the work zone with negative pressure plenums for maximum safety. Air flow is drawn from the room around the operator into the front grille of the cabinet. Moreover the powder coating finish prevents microbial growth on the exterior and delivers a sleek elegant appearance.

Illumination- Fluorescent light illumination greater than 800 lux on work table along with optimal wattage ultra violet UV light ensure high level of cleanliness in our equipments.

Noise level-The biosafety cabinet class II are designed to ensure minimum possible vibration and noise level.

SIZES

900*600*678

1200*600*678

1500*600*678

1800*600*678

Customizable sizes are also available

Available in 2 basic configurations

Table top

Floor standing

Cabinet construction	Stainless steel / PCRC Powder Coated Sheet
Cleanliness class	ISO class 3
Operating Temperature	10-50
Operating relative humidity	<95% (25oC)
Atmospheric pressure	120 Pa
Illumination level	>800
Noise pressure(dB) (A)	<65 db
Air supply filter	Washable
Differential pressure Indicating range	0-500
Differential pressure indicating precision	+ 1%
Power Requirements	500-750 watts

Clinical laboratory processes
Biotechnology
Pharmaceutical industry
General health care sectors
R&D and industrial sectors
Bacteria & virus handling

Frequently Asked Questions

Q1. What is a Biological Safety Cabinet Class II used for?

A Biological Safety Cabinet Class II provides simultaneous protection for laboratory personnel, the sample or product, and the surrounding environment. It is used for handling biohazardous materials, microbial cultures, cell culture work, pharmaceutical compounding, and any application at biosafety levels 1, 2, or 3 that requires both containment and a sterile workspace.

Q2. What is the difference between Biosafety Cabinet Class II Type A1, A2, B1, and B2?

Type A1 does not require external ducting and recirculates 70% of cabinet air. Type A2 must be externally vented and exhausts 70% of air. Type B1 must be externally vented with 70% exhausted and is suitable for work with toxic chemicals in the directly exhausted zone. Type B2 is a total exhaust cabinet with 100% of air externally ducted, designed for the most hazardous chemical and biological work.

Q3. What filtration does a Biosafety Cabinet Class II use?

ACMAS Biosafety Cabinets Class II use HEPA or ULPA filters with 99.99% filtration efficiency for particles as small as 0.3 microns, for both the supply downflow air and the exhaust air, providing triple-barrier protection for personnel, product, and environment.

Q4. What international standards do ACMAS Biosafety Cabinets comply with?

ACMAS Biosafety Cabinets Class II comply with NSF/ANSI 49, EN 12469, JIS K 3800, ISO 14644-1, EN 1822 (H14), IEC 61010-1, and other applicable international standards depending on the type and configuration specified.

Q5. Can ACMAS customize a Biosafety Cabinet Class II to specific requirements?

Yes. ACMAS designs and manufactures Biological Safety Cabinets to exact client specifications including non-standard chamber dimensions, specific interior material grades such as SS-304 or SS-316, particular control interface requirements, custom airflow configurations, and compliance with specific regulatory or international standards.

Q6. Which industries use ACMAS Biosafety Cabinets Class II?

ACMAS Biosafety Cabinets are used in pharmaceutical manufacturing, biotechnology and life sciences, clinical and diagnostic laboratories, hospital pathology departments, research and development centres, government and defence research establishments, and general healthcare and academic institutions.

Q7. Does ACMAS export Biological Safety Cabinets outside India?

Yes. ACMAS exports Biosafety Cabinets Class II to clients in multiple international markets. All units are manufactured to CE and ISO quality standards and can be configured to comply with country-specific regulatory requirements.

Biosafety Cabinet Class II Manufacturers, Exporters & Suppliers in India

Corporate Catalog

Corporate Videos

Product Videos

Exhibition

Product Gallery

Certifications